5FX8
Complete structure of manganese lipoxygenase of Gaeumannomyces graminis and partial structure of zonadhesin of Komagataella pastoris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 226.309, 50.470, 177.622 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 43.163 - 2.600 |
| R-factor | 0.1977 |
| Rwork | 0.195 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fno |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.152 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 62790 | |
| <I/σ(I)> | 7.6 | 3 |
| Completeness [%] | 97.8 | 99 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MES-IMIDAZOLE PH6.5, 20%(V/V) GLYCEROL, 10%(W/V)PEG4000, 0.03M MAGNESIUM CHLORIDE, 0.03M CALCIUM CHLORIDE |
