5FW2
Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-22 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 177.740, 68.240, 188.220 |
| 格子定数 [度] | 90.00, 110.95, 90.00 |
精密化法
| 残基 | 48.251 - 2.676 |
| R因子 | 0.2064 |
| Rwork | 0.205 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.658 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 48.250 | 2.750 |
| 分解能 [Å] (高) | 2.680 | 2.680 |
| Rmerge_l_obs | 0.120 | 0.770 |
| 独立反射数 | 59682 | |
| <I/σ(I)> | 13.2 | 2.2 |
| 完全性 [%] | 99.3 | 91.7 |
| 冗長性 | 7 | 6.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 8.5 | 0.1 M TRIS PH 8.5 0.3M KSCN 15% (W/V) PEG 3400 |
