5FW1
Crystal structure of SpyCas9 variant VQR bound to sgRNA and TGAG PAM target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.637, 66.984, 187.228 |
| Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
| Resolution | 47.622 - 2.499 |
| R-factor | 0.2202 |
| Rwork | 0.218 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.770 |
| Number of reflections | 71152 | |
| <I/σ(I)> | 11.9 | 1.7 |
| Completeness [%] | 99.4 | 98.2 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 0.3 M KSCN, 15% (W/V) PEG 3400 |
