5FVN
X-ray crystal structure of Enterobacter cloacae OmpE36 porin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-24 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.749, 123.260, 116.010 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 115.990 - 1.450 |
| R-factor | 0.15613 |
| Rwork | 0.155 |
| R-free | 0.18308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j1n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.050 |
| Number of reflections | 529555 | |
| <I/σ(I)> | 8 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.4M AMMONIUM SULFATE, 0.1M MES, 10% W/V PEG 3350, PH6.5 |
