5FVL
Crystal structure of Vps4-Vps20 complex from S.cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.140, 53.890, 70.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.696 - 1.973 |
| R-factor | 0.1858 |
| Rwork | 0.183 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v6x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.942 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.160 | 0.520 |
| Number of reflections | 13410 | |
| <I/σ(I)> | 15 | 6.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.3 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.6 | pH 8.6 |
