5FVK
Crystal structure of Vps4-Vfa1 complex from S.cerevisiae at 1.66 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.830, 56.460, 84.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.994 - 1.658 |
R-factor | 0.1857 |
Rwork | 0.184 |
R-free | 0.22230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v6x |
RMSD bond length | 0.006 |
RMSD bond angle | 0.938 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.990 | 1.750 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.100 | 0.610 |
Number of reflections | 22657 | |
<I/σ(I)> | 16.1 | 4.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.4 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | pH 4 |