5FVJ
Crystal structure of TacT (tRNA acetylating toxin) from Salmonella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.400, 85.660, 55.320 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 55.230 - 1.700 |
| R-factor | 0.17447 |
| Rwork | 0.172 |
| R-free | 0.22540 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.524 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.200 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.140 | 0.840 |
| Number of reflections | 32830 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 96.2 | 96.5 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 22 % (W/V) PENTAERYTHRITOL ETHOXYLATE (15/4 EO/OH), 100 MM BIS-TRIS PH6.5, 50 MM AMMONIUM SULPHATE |
