5FVI
Structure of IrisFP in mineral grease at 100 K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.580, 95.940, 139.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.970 - 2.397 |
| R-factor | 0.1612 |
| Rwork | 0.159 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvh |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.760 |
| Number of reflections | 42023 | |
| <I/σ(I)> | 10.93 | 2.58 |
| Completeness [%] | 99.4 | 95.7 |
| Redundancy | 5.53 | 5.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3 M AMMONIUM SULFATE, 100MM BICINE, PH 8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
