5FVC
Structure of RNA-bound decameric HMPV nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 202.010, 233.210, 203.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.190 - 4.170 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DECAMER OF MONOMERIC HMPV N0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.190 | 4.280 |
| High resolution limit [Å] | 4.170 | 4.170 |
| Rmerge | 0.220 | |
| Number of reflections | 36125 | |
| <I/σ(I)> | 9.2 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.5 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS/BICINE, PH 8.5, 90 MM NPS (NAN03, NA2HPO4, (NH4)2SO4), 37.5 % METHYL-2 4-PENTANEDIOL, POLYETHYLENE GLYCOL 1000 AND POLYETHYLENE GLYCOL 3350 (MORPHEUS SCREEN) |
