5FVB
CRYSTAL STRUCTURE OF PHORMIDIUM C-PHYCOERYTHRIN AT PH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 1 |
| Unit cell lengths | 109.165, 109.148, 117.680 |
| Unit cell angles | 78.71, 82.30, 61.55 |
Refinement procedure
| Resolution | 38.891 - 1.930 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aqd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.127 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.890 | 2.030 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 338355 | |
| <I/σ(I)> | 13.4 | 4.8 |
| Completeness [%] | 96.2 | 92 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1M CITRATE (PH 5.0), 5% PEG-6000 AND 25% GLYCEROL |
