5FV8
Structure of cJun-FosW coiled coil complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-08-03 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 63.820, 63.820, 190.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.657 - 1.990 |
| R-factor | 0.2105 |
| Rwork | 0.209 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a02 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.660 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.020 | 0.390 |
| Number of reflections | 13418 | |
| <I/σ(I)> | 19.7 | 2.5 |
| Completeness [%] | 96.2 | 78.4 |
| Redundancy | 17.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.1M SODIUM MALONATE DIBASIC MONOHYDRATE, 0.1M HEPES PH 7.00 AND 5% V/V JEFFAMINE ED-2003. |
