5FV5
KpFlo11 presents a novel member of the Flo11 family with a unique recognition pattern for homophilic interactions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.540, 58.520, 76.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.400 |
R-factor | 0.13596 |
Rwork | 0.134 |
R-free | 0.17937 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uys |
RMSD bond length | 0.012 |
RMSD bond angle | 1.659 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 42777 | |
<I/σ(I)> | 20.95 | 4.7 |
Completeness [%] | 95.9 | 94.7 |
Redundancy | 6.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM ACETATE PH 4.6, 15 % PEG 4000 |