5FV0
The cytoplasmic domain of EssC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-15 |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.917, 152.372, 207.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 122.750 - 2.910 |
R-factor | 0.26202 |
Rwork | 0.261 |
R-free | 0.27484 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.004 |
RMSD bond angle | 0.732 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.630 | 3.070 |
High resolution limit [Å] | 2.910 | 2.910 |
Rmerge | 0.120 | 0.900 |
Number of reflections | 29986 | |
<I/σ(I)> | 21.8 | 4 |
Completeness [%] | 99.0 | 94 |
Redundancy | 12.8 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M SODIUM CACODYLATE PH 6.2, 0.2M CALCIUM ACETATE, 20% (W/V) PEG300 |