5FV0
The cytoplasmic domain of EssC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-15 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.917, 152.372, 207.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 122.750 - 2.910 |
| R-factor | 0.26202 |
| Rwork | 0.261 |
| R-free | 0.27484 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.630 | 3.070 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.120 | 0.900 |
| Number of reflections | 29986 | |
| <I/σ(I)> | 21.8 | 4 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 12.8 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M SODIUM CACODYLATE PH 6.2, 0.2M CALCIUM ACETATE, 20% (W/V) PEG300 |
