5FUI
Crystal structure of the C-terminal CBM6 of LamC a marine laminarianse from Zobellia galactanivorans
Replaces: 4CRRExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.056, 29.523, 51.872 |
Unit cell angles | 90.00, 115.79, 90.00 |
Refinement procedure
Resolution | 46.710 - 1.400 |
R-factor | 0.12787 |
Rwork | 0.126 |
R-free | 0.12704 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uxz |
RMSD bond length | 0.024 |
RMSD bond angle | 2.064 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.700 | 1.430 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.060 | 0.360 |
Number of reflections | 19499 | |
<I/σ(I)> | 10.5 | 2 |
Completeness [%] | 93.0 | 65.3 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 100 MM HEPES PH 7.2, 10 MM MGCL2 AND 27 % POLYACRYLIC ACID. MICROSEEDING WAS ESSENTIAL |