5FUB
Crystal Structure of zebrafish Protein Arginine Methyltransferase 2 catalytic domain with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.070, 147.070, 127.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.405 - 1.997 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.603 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | |
| Number of reflections | 35917 | |
| <I/σ(I)> | 9.2 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 9% PEG 20000, 300MM NACL,100MM MES PH 6.0 AND FOR CRYO IS 10% PEG 20000, 300MM NACL,100MM MES PH 6.0, 15% ETHYLENE GLYCOL |
