5FU4
The complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-16 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.430, 55.190, 44.390 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 40.320 - 2.000 |
| R-factor | 0.23825 |
| Rwork | 0.236 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aot |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.632 |
| Data reduction software | xia2 (3DII) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 13038 | |
| <I/σ(I)> | 7.7 | 1.9 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% W/V PEG 1500 |
