5FU3
The complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Collection date | 2015-09-20 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.914, 54.753, 43.986 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 43.840 - 1.610 |
| R-factor | 0.19837 |
| Rwork | 0.195 |
| R-free | 0.25968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aot |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.840 | 1.640 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.040 | 0.670 |
| Number of reflections | 24439 | |
| <I/σ(I)> | 17.5 | 1.6 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 MM NA FORMATE 20MM NH4 ACETATE 20 MM NA3 CITRATE 20 MM NAK TARTRATE (RACEMIC) 20 MM NA OXAMATE 50 MM IMIDAZOLE 50 MM MES PH 6.5 12.5% MPD 12.5% PEG 1000 12.5% PEG 3350 |
