5FU2
The complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.623, 47.272, 83.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.980 - 1.400 |
| R-factor | 0.16019 |
| Rwork | 0.158 |
| R-free | 0.19976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aot |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.980 |
| Number of reflections | 36544 | |
| <I/σ(I)> | 17.4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM CACL2 100 MM NA ACETATE PH 5.0 20% W/V PEG 6000 |
