5FU2
The complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-31 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.623, 47.272, 83.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.980 - 1.400 |
R-factor | 0.16019 |
Rwork | 0.158 |
R-free | 0.19976 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5aot |
RMSD bond length | 0.010 |
RMSD bond angle | 1.636 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.270 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.060 | 0.980 |
Number of reflections | 36544 | |
<I/σ(I)> | 17.4 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 200 MM CACL2 100 MM NA ACETATE PH 5.0 20% W/V PEG 6000 |