5FTW
Crystal structure of glutamate O-methyltransferase in complex with S- adenosyl-L-homocysteine (SAH) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.580, 72.870, 81.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.460 - 1.800 |
| R-factor | 0.17948 |
| Rwork | 0.176 |
| R-free | 0.23699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bc5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.580 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.150 | 0.600 |
| Number of reflections | 23011 | |
| <I/σ(I)> | 8.1 | 2.3 |
| Completeness [%] | 96.1 | 83.4 |
| Redundancy | 4.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE TRIHYDRATE |
