5FTD

Crystal structure of Pif1 helicase from Bacteroides apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-04-30
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	66.783, 70.747, 103.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.564 - 1.695
R-factor	0.1695
Rwork	0.168
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftb
RMSD bond length	0.012
RMSD bond angle	1.274
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.490
Number of reflections	48257
<I/σ(I)>	25	2.22
Completeness [%]	87.0	46
Redundancy	6.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M BIS-TRIS PROPANE PH 6.5 0.1 CALCIUM ACETATE 10% PEG 4000