5FTD
Crystal structure of Pif1 helicase from Bacteroides apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.783, 70.747, 103.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.564 - 1.695 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.19350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ftb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.490 |
| Number of reflections | 48257 | |
| <I/σ(I)> | 25 | 2.22 |
| Completeness [%] | 87.0 | 46 |
| Redundancy | 6.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PROPANE PH 6.5 0.1 CALCIUM ACETATE 10% PEG 4000 |
