5FT3
Aedes aegypti GSTe2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.811, 86.563, 101.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.745 - 1.431 |
| R-factor | 0.1359 |
| Rwork | 0.134 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zml |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.960 |
| Data reduction software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.850 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 78165 | |
| <I/σ(I)> | 18.8 | 2.1 |
| Completeness [%] | 98.0 | 80.7 |
| Redundancy | 5.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M NACL, 0.1 M BIS-TRIS PH 5.7, 25 % PEG 3350 |
