5FT0
Crystal structure of gp37(Dip) from bacteriophage phiKZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.040, 65.020, 85.400 |
| Unit cell angles | 90.00, 107.68, 90.00 |
Refinement procedure
| Resolution | 46.025 - 2.200 |
| R-factor | 0.1952 |
| Rwork | 0.194 |
| R-free | 0.21890 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.655 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX (AUTOSOLVE) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.600 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.810 |
| Number of reflections | 38346 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM KH2PO4, 100 MM NAH2PO4, 100 MM MES PH 6.0, 800 MM NACL, 0.2 M SODIUM THIOCYANATE |
