5FSG
Structure of the hantavirus nucleoprotein provides insights into the mechanism of RNA encapsidation and a template for drug design
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.630, 86.840, 131.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.805 - 3.209 |
| R-factor | 0.2909 |
| Rwork | 0.290 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4exk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.810 | 3.290 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.110 | 0.720 |
| Number of reflections | 25102 | |
| <I/σ(I)> | 13.79 | 1.83 |
| Completeness [%] | 99.6 | 96.2 |
| Redundancy | 5.9 | 2.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 17% PEG3350 0.2M AMMONIUM CITRATE PH 5.0 |
