5FSC
Structure of tectonin 2 from Laccaria bicolor in complex with allyl- alpha_4-methyl-mannoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-29 |
| Detector | RAYONIX |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 69.007, 69.007, 348.953 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.770 - 1.950 |
| R-factor | 0.14752 |
| Rwork | 0.146 |
| R-free | 0.18476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fsb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.711 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.770 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.140 | 0.410 |
| Number of reflections | 24046 | |
| <I/σ(I)> | 12.6 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.3M MAGNESIUM SULFATE 0.1M MES PH 6.5 80% LITHIUM FORMATE WAS USED AS CROPROTECTANT |
