5FS7
Crystal structure of the G262S mutant of human apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-09-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.410, 114.570, 120.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 114.530 - 1.850 |
| R-factor | 0.18072 |
| Rwork | 0.179 |
| R-free | 0.21691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fs8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.130 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.120 | 1.040 |
| Number of reflections | 106911 | |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 98.6 | 97.4 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M LITHIUM SULFATE, 0.1M TRIS, PH 8.5, AND 25% POLY-ETHYLENE GLYCOL 3350 |
