5FRY
crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR with 3,5-dimethylphenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 287 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.730, 70.696, 105.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.191 - 1.792 |
R-factor | 0.268 |
Rwork | 0.265 |
R-free | 0.31990 |
Structure solution method | MIR |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.079 |
Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.191 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.680 |
Number of reflections | 33213 | |
<I/σ(I)> | 55.11 | 2.33 |
Completeness [%] | 99.4 | 99 |
Redundancy | 13.8 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |