5FRW
crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR with Phenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.946, 70.475, 104.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.064 - 2.100 |
| R-factor | 0.1997 |
| Rwork | 0.195 |
| R-free | 0.24620 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.166 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 25436 | |
| <I/σ(I)> | 37.65 | 1.86 |
| Completeness [%] | 98.8 | 88.5 |
| Redundancy | 6.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
