5FRS

Structure of the Pds5-Scc1 complex and implications for cohesin function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	187.471, 248.461, 62.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.378 - 4.073
R-factor	0.2204
Rwork	0.219
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.844
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.700
Rmerge	0.020
Number of reflections	29319
<I/σ(I)>	8.39	1.1
Completeness [%]	99.2
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1