5FRR

Structure of the Pds5-Scc1 complex and implications for cohesin function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	I 4 2 2
Unit cell lengths	283.690, 283.690, 172.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 5.800
R-factor	0.249
Rwork	0.249
R-free	0.31110
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.593
Data reduction software	XDS
Data scaling software	XDS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	5.800	5.800
Rmerge	0.020
Number of reflections	10082
<I/σ(I)>	17.1	1.57
Completeness [%]	99.3
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1