5FRO
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI XZ446 (compound 4f)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-19 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.900, 159.900, 123.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.533 - 2.670 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bdz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.618 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.530 | 2.770 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.080 | 1.000 |
Number of reflections | 44515 | |
<I/σ(I)> | 14.4 | 2 |
Completeness [%] | 96.6 | 98 |
Redundancy | 14.4 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M AMMONIUM SULFATE, 25% (V/V) GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 1MM EDTA, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |