5FRE
Characterization of a novel CBM from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-28 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.348, 76.714, 67.254 |
Unit cell angles | 90.00, 93.46, 90.00 |
Refinement procedure
Resolution | 40.610 - 1.900 |
R-factor | 0.17471 |
Rwork | 0.173 |
R-free | 0.20874 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fra |
RMSD bond length | 0.014 |
RMSD bond angle | 1.718 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.690 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.390 |
Number of reflections | 50882 | |
<I/σ(I)> | 13.2 | 3 |
Completeness [%] | 98.8 | 93.4 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 0.1M CH3COONA PH 4.5, 0.2M (CH3CHOO)2CA, 8% (PEG 550 MME AND PEG 20,000) |