5FRA
CBM40_CPF0721-6'SL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.502, 84.267, 89.996 |
| Unit cell angles | 90.00, 90.79, 90.00 |
Refinement procedure
| Resolution | 39.690 - 2.000 |
| R-factor | 0.18011 |
| Rwork | 0.179 |
| R-free | 0.21035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v73 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.690 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 74668 | |
| <I/σ(I)> | 11.8 | 3.1 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 25% PEG 2K MME 0.3M CH3COONA 0.1M CH3COONA PH4.5 |
