5FQN
Crystal structure of M. musculus protein arginine methyltransferase PRMT6 with SAH at 1.65 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 |
| Unit cell lengths | 79.426, 79.426, 118.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.772 - 1.657 |
| R-factor | 0.1793 |
| Rwork | 0.178 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c08 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.660 |
| Rmerge | 0.080 | 1.724 |
| Number of reflections | 43482 | |
| <I/σ(I)> | 10.1 | 0.8 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS PH 8.0 200 MM MGCL2 12% PEG 6000 |
