5FQ9

Crystal structure of the OXA10 with 1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-27
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	48.898, 103.109, 125.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.424 - 1.500
R-factor	0.158
Rwork	0.157
R-free	0.18060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k6r
RMSD bond length	0.014
RMSD bond angle	1.344
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.440	1.120
High resolution limit [Å]	1.500	1.100
Rmerge	0.050	1.100
Number of reflections	253261
<I/σ(I)>	5.6	2
Completeness [%]	99.9	99.5
Redundancy	5.9	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		0.2M NACL, 0.1M NA ACETATE PH 5.0, 20% PEG 6000