5FQ8
Crystal structure of the SusCD complex BT2261-2264 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-31 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.110, 122.743, 122.830 |
| Unit cell angles | 111.41, 98.33, 98.51 |
Refinement procedure
| Resolution | 52.933 - 2.750 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 5FQ4 3h3i 1fep |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.942 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.950 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.190 | 0.660 |
| Number of reflections | 125711 | |
| <I/σ(I)> | 5.8 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM FORMATE DIHYDRATE 0.05 M TRIS PH 8 18-22% PEG3350 |
