5FQ7

Crystal structure of the SusCD complex BT2261-2264 from Bacteroides thetaiotaomicron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-31
Detector	DECTRIS PIXEL
Spacegroup name	P 21 21 21
Unit cell lengths	123.591, 180.159, 245.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.432 - 3.400
R-factor	0.206
Rwork	0.201
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 5FQ4 5fq3
RMSD bond length	0.012
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.450	3.470
High resolution limit [Å]	3.400	3.400
Rmerge	0.170	1.030
Number of reflections	76187
<I/σ(I)>	10.3	2
Completeness [%]	100.0	100
Redundancy	7.5	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M MAGNESIUM FORMATE DIHYDRATE 0.05 M TRIS PH 8 18-22% PEG3350