5FQ6
Crystal structure of the SusCD complex BT2261-2264 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-04 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 222.027, 91.941, 261.219 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 48.799 - 2.800 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 5FQ4 AND 5FQ3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.200 | 1.310 |
| Number of reflections | 256052 | |
| <I/σ(I)> | 8.9 | 2.1 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 9.3 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM FORMATE DIHYDRATE 0.05 M TRIS PH 8 18-22% PEG3350 |
