5FOW
HUMANISED MONOMERIC RADA IN COMPLEX WITH WHTA TETRAPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.479, 87.598, 61.938 |
| Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
| Resolution | 33.232 - 1.797 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b3b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.808 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.230 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 38505 | |
| <I/σ(I)> | 9.96 | 3.05 |
| Completeness [%] | 95.7 | 93.5 |
| Redundancy | 2.79 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
