5FOV
HUMANISED MONOMERIC RADA IN COMPLEX WITH FHTG TETRAPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.334, 87.233, 61.543 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 43.617 - 1.739 |
| R-factor | 0.1781 |
| Rwork | 0.176 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b3b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.490 | 1.840 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.120 | 0.460 |
| Number of reflections | 42203 | |
| <I/σ(I)> | 10.07 | 2.75 |
| Completeness [%] | 96.2 | 83.9 |
| Redundancy | 3.56 | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
