5FOS
HUMANISED MONOMERIC RADA IN COMPLEX WITH OLIGOMERISATION PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.790, 61.780, 87.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.949 - 1.350 |
| R-factor | 0.1434 |
| Rwork | 0.141 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.950 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 47394 | |
| <I/σ(I)> | 16.33 | 2.65 |
| Completeness [%] | 96.3 | 98.6 |
| Redundancy | 4.9 | 4.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
