5FOO
6-phospho-beta-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.017, 109.899, 158.500 |
| Unit cell angles | 90.00, 92.02, 90.00 |
Refinement procedure
| Resolution | 158.400 - 2.100 |
| R-factor | 0.17625 |
| Rwork | 0.174 |
| R-free | 0.21989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b3k |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 205534 | |
| <I/σ(I)> | 12.6 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.33 MM SPY1599 WAS REACTED WITH 4.2 MM 6-PHOSPHO-CYCLOPHELLITOL EPOXIDE IN THE BUFFER OF 50 MM HEPES AND 150 MM NACL, PH 7.0, BEFORE BEING MIXED WITH THE SAME VOLUME OF BUFFER OF 0.1 M BIS TRIS PROPANE PH7.5, 20% PEG 3350 AND 0.15 M NABR |
