5FOK
Crystal structure of the siderophore receptor PiuA from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-10 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 194.010, 132.970, 73.590 |
Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
Resolution | 71.930 - 1.900 |
R-factor | 0.18955 |
Rwork | 0.188 |
R-free | 0.21058 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fcp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.217 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.930 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.730 |
Number of reflections | 142771 | |
<I/σ(I)> | 13.7 | 2.5 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 32% PEG 400, 0.05 M TRIS PH 8.5, 0.05 M SODIUM SULPHATE AND 0.05 M LICL2 |