5FNO
Manganese Lipoxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.725, 111.374, 171.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.705 - 2.038 |
| R-factor | 0.1701 |
| Rwork | 0.168 |
| R-free | 0.21330 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.120 | 0.630 |
| Number of reflections | 81415 | |
| <I/σ(I)> | 17.9 | 3.9 |
| Completeness [%] | 98.1 | 86 |
| Redundancy | 18.8 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M AMMONIUM CITRATE DIBASIC 12% PEG-3350 |
