5FNN
Iron and Selenomethionine containing Iron sulfur cluster repair protein YtfE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-29 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 41 |
Unit cell lengths | 98.620, 98.620, 125.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.580 - 2.090 |
R-factor | 0.21201 |
Rwork | 0.211 |
R-free | 0.23050 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.544 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.900 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.040 | 0.440 |
Number of reflections | 34895 | |
<I/σ(I)> | 19.37 | 2.67 |
Completeness [%] | 99.3 | 95.4 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M TRISCL PH8.5, 21% PEG4000, 0.2 M MGCL2, pH 7.5 |