5FNB
CRYSTAL STRUCTURE OF FUNGAL VERSATILE PEROXIDASE FROM PLEUROTUS ERYNGII SEPTUPLE MUTANT E37K, H39R, V160A, T184M, Q202L, D213A & G330R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-06 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.060, 106.010, 107.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.015 - 1.792 |
R-factor | 0.1862 |
Rwork | 0.185 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fmu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.104 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.410 | 1.500 |
Number of reflections | 59335 | |
<I/σ(I)> | 6.8 | 0.7 |
Completeness [%] | 98.6 | 94.2 |
Redundancy | 13.1 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M TRIS-HCL (PH 7.8), 5 % PEG3350, 1.25 M (NH4)2SO4 |