5FMT
CrIFT54 CH-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 57.570, 145.460, 147.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.365 - 1.878 |
| R-factor | 0.1995 |
| Rwork | 0.197 |
| R-free | 0.24470 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.360 | 1.990 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.170 | 0.940 |
| Number of reflections | 25144 | |
| <I/σ(I)> | 11.16 | 1.73 |
| Completeness [%] | 98.2 | 90.9 |
| Redundancy | 4.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM TRIS-HCL PH 8.3, 2% MPD, 80 MM AMMONIUM SULFATE, 30% PEG5000, 5% GLYCEROL |
