5FMS
mmIFT52 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 204.910, 204.910, 204.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.298 - 3.488 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fmr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.602 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 3.610 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.380 | 1.500 |
| Number of reflections | 35358 | |
| <I/σ(I)> | 11.29 | 1.65 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 21 | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM BIS-TRIS PH5.5, 1.8 M AMMONIUM SULFATE |
