5FMI
Human Bak Q77L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.024, 54.528, 58.716 |
| Unit cell angles | 90.00, 115.50, 90.00 |
Refinement procedure
| Resolution | 26.187 - 1.491 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ims |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.705 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.190 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.030 | 0.160 |
| Number of reflections | 26752 | |
| <I/σ(I)> | 26.6 | 7.5 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 10% PEG 3350, 0.1M SODIUM ACETATE PH 4.5, 20 MM ZINC ACETATE |
