5FMC
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructose and BIS-TRIS propane buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.432, 204.740, 145.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.630 - 1.840 |
| R-factor | 0.16426 |
| Rwork | 0.163 |
| R-free | 0.17907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ann |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.630 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.340 |
| Number of reflections | 192642 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.3M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M BIS-TRIS PROPANE PH 7.5 |
