5FMC
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructose and BIS-TRIS propane buffer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.432, 204.740, 145.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 118.630 - 1.840 |
R-factor | 0.16426 |
Rwork | 0.163 |
R-free | 0.17907 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ann |
RMSD bond length | 0.007 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.630 | 1.870 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.060 | 0.340 |
Number of reflections | 192642 | |
<I/σ(I)> | 7.4 | 1.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.3M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M BIS-TRIS PROPANE PH 7.5 |