5FM6
Double-heterohexameric rings of full-length Rvb1(ADP)Rvb2(apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 209.610, 209.610, 137.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.635 - 2.997 |
| R-factor | 0.2312 |
| Rwork | 0.230 |
| R-free | 0.25610 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.090 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 1.500 |
| Number of reflections | 45338 | |
| <I/σ(I)> | 15.4 | 1.2 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 9.8 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 5.7; 1.8 M AMMONIUM SULPHATE |
